Image and numerical data detailing interactions between metal reducing bacteria and metals (NERC grant NE/J024732/1)

Dataset description

These data show images recorded using a variety of methods of a model system of bacterial metal reduction. In all cases the bacteria grew from a pure culture of Geobacter sulfurreducens, and grew undisturbed on thin films of amorphous Fe oxyhydroxide ? ferrihydrite. The different imaging methodologies have highlighted different features of this interaction. AFM shows the surface texture of the bacteria and ferrihydrite films; epifluorescence was used to allow counting of the cells at different time points from 0 to 12 days post inoculation (cell counts available in excel spreadsheet); and confocal imaging allow visualisation of the redox patterns surrounding cells and to identify areas of bioreduced Fe(II) (quantification of Fe(II) available in excel spreadsheet). ? The following data is included: ? 9 x AFM images of Geobacter sulfurreducens bacteria growing on ferrihydrite films ? 5 x epifluorescence images of Geobacter sulfurreducens bacteria growing on ferrihydrite films over time ? spreadsheet bacterial counts associated with epifluorescence images ? 7 x confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with redox green staining of appendages ? 5 x example confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with Fe(II) highlighted by RhoNox-1 ? Spreadsheet of quanitfication of RhoNox intensity against bacteria and Fe co-location Data is presented which shows the formation of precious metal nanoparticles on the surface of geobacter sulfurreducens cells. The images were produced by CryoTEM. Full details of the experiment are available in this publication http://onlinelibrary.wiley.com/doi/10.1002/ppsc.201600073/full ? Powerpoint presentation of TEM images of precious metal nanoparticles formed on the surface of Geobacter cells

Constraints

Much of the data is still unpublished, but submitted and under review, so we expect it to be in the public domain by the end of the embargo, 1st November 2018.

Further information

For more information please contact:

Enquiries

Environmental Science Centre, Nicker Hill, Keyworth
Nottingham
NG12 5GG

Tel : +44 (0)115 936 3143
Fax :+44 (0)115 936 3276
Email :enquiries@bgs.ac.uk

 

Dataset details

Author(s) Not available
Principal Investigator(s) Not available
Language English
Curator British Geological Survey
Supply media/format Not available
Storage format Not available
Frequency of update not applicable
Start of capture {ts '2014-03-01 00:00:00'} Not known
End of capture {ts '2016-06-01 00:00:00'} June 2016
Contact details
Department Enquiries
Organisation British Geological Survey
Address Environmental Science Centre, Nicker Hill, Keyworth
City Nottingham
County Nottinghamshire
Country United Kingdom
Postcode NG12 5GG
E-mail enquiries@bgs.ac.uk
Telephone +44 (0)115 936 3143
Fax +44 (0)115 936 3276
Keywords
Topic category code (ISO) geoscientificInformation (information pertaining to earth sciences)
Keywords CHEMICAL ANALYSIS
MICROBIOLOGICAL PROCESSES
MICROSCOPY
SIMULATION
Keyword source BGS Keyphrases
Spatial details
Spatial Reference System Not available
Dataset extent
Coverage (Lat/Long) North boundary : 
East boundary  : 
South boundary : 
West boundary  : 
Metadata
Metadata language English
Metadata last updated 1st June 2017
Metadata standard compliance NERC profile of ISO19115:2003
Copyright and IPR
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.