QICS Data: Gene expression data of scallops Pecten maximus and mussels Mytilus edulis held in the vicinity of a sub-seabed CO2 release (2012)

Dataset description

The QICS project (Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbons Storage) was established to improve our understanding of the potential impacts of CO2 release on the environment and to develop tools and best practice for monitoring sub-seabed CCS reservoirs. To monitor the potential impact of a CO2 leak to surficial benthic megafauna, cages of bivalves (the common mussel Mytilus edulis Linnaeus, 1758 and the king scallop Pecten maximus (Linnaeus, 1758)) were deployed at the gas release site and at a reference site in the QICS experiment - both within Ardmucknish Bay, Oban, Scotland. Replicate individuals were sampled at six time points over a 125-day period, which spanned both the 37-day injection and recovery phases of the experiment, in order to establish impacts to molecular physiology. Samples of bivalves were also simultaneously sampled from a reference site within the bay in order to contrast changes in physiology induced by the gas release with naturally variability in the physiological performance of both species. There was no evidence of gene regulation of either selected carbonic anhydrases (CAx genes) or the alpha subunit of sodium potassium ATPAses (ATP1A genes) in individual bivalves collected from the CO2 gas release site, in either species. In the common mussel Mytilus edulis there was only evidence for changes with time in the expression of genes coding for different classes of carbonic anhydrase. It was concluded that the effects of the plume of elevated pCO2 on ion-regulatory gene transcription were negligible in both species. Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.

Further information

For more information please contact:

Enquiries

Environmental Science Centre, Nicker Hill, Keyworth
Nottingham
NG12 5GG

Tel : +44 (0)115 936 3143
Fax :+44 (0)115 936 3276
Email :enquiries@bgs.ac.uk

Associated dataset(s)

NERC Project: QICS - Quantifying and monitoring environmental impacts of geological carbon storage (2010 - 2014)

Dataset details

Author(s) Chris Hauton
Principal Investigator(s) Chris Hauton
National Oceanography Centre Southampton
Language English
Curator British Geological Survey
Supply media/format .xls; Version:N/A
Storage format Not available
Frequency of update not applicable
Start of capture {ts '2012-05-08 00:00:00'} Not known
End of capture {ts '2012-09-09 00:00:00'} 9th September 2012
Online access URL  
Lineage statement Peer-reviewed paper to be published in the International Journal of Greenhouse Gas Control (in press). Will include original gene expression values and details of processing (citation to follow). Organism sampling, including tissue dissections conducted by Chris Hauton and Elizabeth Morgan; Ocean and Earth Science, University of Southampton Molecular methods conducted by Nicola Pratt; Ocean and Earth Science, University of Southampton This spreadsheet compiled by Hauton and Pratt, Ocean and Earth Science, University of Southampton. RNA extraction using TRIreagent (SigmaAldrich, Dorset, UK) according to manufacturer's instructions (Product No. T9424) RNA pellets air dried and diluted in DEPC-treated water (DEPC = diethyl pyrocarbonate) RNA purity assessed by measuring absorbance at 230nm, 260nm and 280nm using a NanoDrop spectrophotometer RNA quantity assessed using Bio-Rad (Herts, UK) Experion electrophoresis system according to manufacturer's instructions (Product No. 700-7104) RNA integrity (RQI) assessed using Bio-Rad Experion electrophoresis system according to manufacturer's instructions RNA DNase-treated using Promega (Hants, UK) RQ1 RNase-Free DNase, according to manufacturer's instructions (Product No. M6101) cDNA produced using LifeTechnologies (Strathclyde, UK) Superscript III Reverse Transcriptase according to manufacturer's instructions with oligo-dT priming (Product No. 18080044) qPCR carried out using a Qiagen (West Sussex) Rotorgene 3000 instrument qPCR Mastermix - Precision SY Mastermix with SYBR green (PrimerDesign, Hants, UK) (Product No. Precision-SY). qPCR conditions: 95 degrees for 10 minutes; 40 cycles of 95 degrees for 10 seconds, 60 degrees for 60 seconds qPCR amplicon specificity verified by melt curve analysis qPCR run files available upon request to Chris Hauton (ch10@noc.soton.ac.uk) Acknowledgements: field sampling and molecular lab work funded under the UK NERC QICS Project (NE/H013881/1)
Supplementary information Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.
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Contact details
Department Enquiries
Organisation British Geological Survey
Address Environmental Science Centre, Nicker Hill, Keyworth
City Nottingham
County Nottinghamshire
Country United Kingdom
Postcode NG12 5GG
E-mail enquiries@bgs.ac.uk
Telephone +44 (0)115 936 3143
Fax +44 (0)115 936 3276
Keywords
Topic category code (ISO) geoscientificInformation (information pertaining to earth sciences)
Keywords CARBON
ENVIRONMENTAL IMPACT
MONITORING
STORAGE
Keyword source BGS Keyphrases
Spatial details
Spatial Reference System Geographical, topographical or geological place names and locations, including postal address
Dataset extent
Coverage (Lat/Long) North boundary : 56.49
East boundary  : -5.42
South boundary : 56.49
West boundary  : -5.42
Metadata
Metadata language English
Metadata last updated 12th August 2015
Metadata standard compliance NERC profile of ISO19115:2003
Copyright and IPR
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.